Identification of New Chk2 Substrates

Abstract

Human Chk2 protein kinase is involved in DNA damage checkpoint pathway. After DNA damage Chk2 is phosphorylated at multiple sites in N-terminal SCD (SQ/TQ cluster domain) and activated. We reported here that Chk2 phosphorylated at T6S and T26/S28 is localized to the centrosome and the midbody. During mitosis we observed kinetochore localization of Chk2 phosphorylated at T26/S28. In attempt to find interactions between Chk2 and proteins with similar cellular localization, we found that Chk2 physically interacts with Plkl. Immunofluorescence has shown co- localization of phosphorylated Chk2 and Plkl to the centrosome in early mitosis and to the midhody in late mitosis. We conclude that cellular localization of phosphoryalted Chk2 and its interaction with Plkl are signs for a possible involvement in lateral communications between DNA damage and mitotic checkpoints.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2002
Accession Number
ADA410391

Entities

People

  • Lyuben Tsvetkov

Organizations

  • Yale University

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  • Amino Acids
  • Breast Cancer
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Fields of Study

  • Biology

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