Fibrinolysis in Tumor Associated Angiogenesis

Abstract

Tumor-associated blood vessels are abnormally leaky and allow the extravasation of fibrinogen and other components of the coagulation system. Exposure of these procoagulants to extravascular matrix components and procoagulant tumor products activates the coagulation cascade and deposits large amounts of fibrin. Therefore, in tumors, angiogenic endothelial proteolytic capability would have to include fibrinolysis. We have isolated pure populations of tumor endothelial cells from xenograft breast tumors and from mammary fat pads of nude mice. RNA obtained from tumor- and mammary fat pad-associated endothelial cells was used to synthesize cDNA and cDNA libraries, which were used in differential cloning techniques to compare gene expression in tumor-associated endothelial cells with that of mammary fat pad endothelial cells.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2002
Accession Number
ADA410455

Entities

People

  • Sandra W. Mcleskey

Organizations

  • University of Maryland, Baltimore

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Angiogenesis
  • Biomedical Research
  • Blood
  • Blood Coagulation Factors
  • Blood Vessels
  • Breast Cancer
  • Central Nervous System
  • Endothelial Cells
  • Fibrinolysis
  • Gene Expression
  • Genetic Structures
  • Mammary Glands
  • Neoplasms
  • Nervous System
  • Polymerase Chain Reaction
  • Proteins

Readers

  • Molecular Biology and Genetics
  • Trauma Surgery or Emergency Medicine.