Fourier Interferometry/Spectral Imaging of Response to Metabolic Challenges in Perinuclear Mitochondria of Normal and Malignant Cells
Abstract
The problem of mitochondrial responses in sequence to metabolic challenges continues to be a puzzling one. While cytoplasmic and nuclear (i.e. extra-mitochondrial) responses to glucose and glycolytic intermediates are rather regular and reproducible, notably in ATP-depleted cells, mitochondrial responses remain erratic and irregular. There are, however, striking changes in mitochondrial distribution, intracellular organization and morphology which are associated with cell growth conditions, differentiation and transformation. Thus, a study of mitochondrial morphology and distribution is first attempted with vital probes such as DASPMI, Mitotracker Green (TM) and TMRE. The acquisition of structural and functional information on mitochondria of living normal and malignant cells can be enhanced by introduction of new methods using Fourier interferometry and spectral imaging.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 25, 2001
- Accession Number
- ADA410465
Entities
People
- Ceren Ornek
- Elli Kohen
- John Berry
- Joseph G. Hirschberg
- Marco Monti
Organizations
- University of Miami