Refined Calibration Methods for Optical Potential Mapping in Single Cardiomyocytes

Abstract

Fluorescence imaging provides a comprehensive view of the electrical behavior of excitable cells, the relative nature of the optical signals however requires additional means for calibration. The determination of the actual electrical field strength is subject to uncertainty even when using concurrent electrode measurements. A combined approach of electrical field measurement and optical mapping of the spatially distributed polarizations provides a satisfying calibration of the optical signals in our field-stimulation experiments.

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Document Details

Document Type
Technical Report
Publication Date
Oct 25, 2001
Accession Number
ADA410518

Entities

People

  • D. Dapra
  • D. Platzer
  • H. Windisch

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Amplitude
  • Calibration
  • Classification
  • Detectors
  • Electromagnetic Wave Detectors
  • Fluorescence
  • Intensity
  • Light Sources
  • Linearity
  • Membrane Potentials
  • Membranes
  • Military Research
  • Optical Detectors
  • Photodetectors
  • Photodiodes
  • Polarization

Fields of Study

  • Physics

Readers

  • Computational Modeling and Simulation
  • Electrochemical Engineering/ Fuel Cell Technologies
  • Image Processing and Computer Vision.