New Strategy for the Redirection of Cytolytic T Lyphocytes to Breast Tumors

Abstract

The main objective of this research project has been to apply the T-body approach for the immunotherapy of breast cancer. To this end, we endowed human lymphocytes with anti-erbB2 specificity by transducing them with a tripartite chimeric receptor made of anti-erbB2 scFv-linked through the homeo-domain, trans membrane and endo-domain of cD28 to the cytoplasmic region of the FcR gamma chain. We report on the procedures and protocol developed to construct the optimal configuration of receptor and a protocol for efficient chimeric gene expression in human T cells.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2002
Accession Number
ADA410523

Entities

People

  • Zelig Eshhar

Organizations

  • Weizmann Institute of Science

Tags

DTIC Thesaurus Topics

  • Biomedical And Dental Materials
  • Blood
  • Blood Cells
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Gene Expression
  • Lymphocytes
  • Neoplasms
  • Polymeric Films
  • Stem Cells
  • T Lymphocytes
  • Thymocytes
  • Tissues
  • Tumor Cell Line

Readers

  • Immunology
  • Systems Analysis and Design

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech