New Triterpenoids for Prevention of Breast Cancer

Abstract

We have shown that the synthetic triterpenoid, 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid (CDDO) is a highly potent inhibitor of the proliferation of several ER-positive and ER-negative human breast cancer cell lines. Furthermore, CDDO at nanomolar levels will block de novo synthesis of two inflammatory enzymes that have been implicated in the carcinogenic process, namely inducible nitric oxide synthase (iNOS) and inducible cyclooxygenase (COX-2). During the period of funding of this grant, we have attempted to use CDDO in vivo to prevent experimental breast cancer in rats, but we have not been successful thus far, in spite of the extremely high potency of CDDO as a suppressor of the growth of numerous breast cancer cells in culture. We attribute this poor activity in vivo to inadequate pharmacokinetic properties, so therefore we have undertaken the synthesis of a large number of derivatives of CDDO, which are esters, amides, and imidazolides, relatively easily made from CDDO itself. We have evaluated many of these new structures in cell culture; they are highly active as suppressors of synthesis of iNOS and as inhibitors of growth of many tumor cells in culture, including human breast cancer cells. Evaluation of these new agents in vivo is a future goal.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2002
Accession Number
ADA410760

Entities

People

  • Michael B. Sporn

Organizations

  • Dartmouth College

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cancer
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Medical Personnel
  • Organic Chemistry
  • Peptide Growth Factors
  • Proteins

Fields of Study

  • Chemistry

Readers

  • Breast cancer cell signaling and growth regulation.
  • Immunology and Pathology
  • Molecular and Cellular Biochemistry