Investigation of the Predictability of Neural Cell Survival After Exposure to Non-Uniform Electric Fields

Abstract

Cortical rat neurons were trapped by negative dielectrophoresis (DEP) using a planar quadruple electrode structure. The nonuniform field created by this structure was calculated using a finite element software package. By representing the neuron with a single-shell model the membrane potential induced by the electric field can be estimated. It was investigated whether the physiological state of the trapped neurons can be predicted using this estimation. Experimentally, the physiological state of cortical cells trapped at different amplitudes and frequencies of the input signal was determined using a staining method. The authors conclude that a rough estimate of the minimum frequency and maximum amplitude can be given to predict the status of viability of the cells after being dielectrophoretically trapped.

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Document Details

Document Type
Technical Report
Publication Date
Oct 25, 2001
Accession Number
ADA410768

Entities

People

  • E. Marani
  • T. Heida
  • W. L. C. Rutten

Organizations

  • University of Twente

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amplitude
  • Biological Staining And Labeling
  • Biomedical Technology
  • Cell Physiological Processes
  • Cells
  • Diameters
  • Electric Fields
  • Electrical Engineering
  • Electrodes
  • Engineering
  • Finite Element Analysis
  • Frequency
  • Images
  • Membrane Potentials
  • Membranes
  • Relaxation Time
  • Signal Generators

Readers

  • Computational Modeling and Simulation
  • Neural Network Machine Learning.
  • Plasma Physics / Magnetohydrodynamics