Characterization of Putative Homeostatic Molecules in Prostate Development and Androgen-Independent Prostate Cancer
Abstract
The mortality associated with prostate cancer (PCa) is mostly due to the recurrent of an androgen-independent (Al) PCa. It is believed that AIPCa may derive from stem (or basal) cell population of prostate gland with a malignant phenotype. However, the characterization of stem cell in prostate gland is still lacking. Therefore, the main goal of this project is to define the phenotype of stem cell in normal prostate gland and the molecule(s) involved in maintaining its normal phenotype. In this grant, we are proposing to isolate different basal cell population of prostatic epithelia from normal prostate gland and document the molecular markers during the differentiation lineage of these cells. currently, we have isolated several clones derived from basal cell population of prostate gland with various differentiation potentials. Using a three-dimension cell culture system, we were able to observe their different cell polarization abilities. On the other hand, we have delineated the biologic function and gene regulation two unique molecules (i.e., DOC-2 and DIPl/2) isolated from normal basal cell of prostatic epithelium in AIPCa. The outcome of these results could have a significant impact on understanding the biology of AIPCa and developing new target for PCa therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2002
- Accession Number
- ADA410785
Entities
People
- Jer-Tsong Hsieh
Organizations
- University of Texas Southwestern Medical Center