Thromboxane Synthase and Prostate Cancer Progression
Abstract
The initiation and progression of prostate cancer remain not well understood to enable rational development of interventional therapy. Thromboxane synthase is an enzyme downstream cyclooxygenase, utilizing prostaglandin H to form thromboxane A2. Using immunohistochemistry analysis, we found that 25% of clinical prostate tumor specimens had strong expression of thromboxane synthase; 33% of cases had medium expression and 42% of cases had weak expression of thromboxane synthase. Prostate cancer cells isolated from lymph node metastasis had higher levels of thromboxane synthase expression and activity than those isolated from the primary tumor sites in an animal model. We cloned and sequenced full-length thromboxane synthase cDNA from PC-3 cells and constructed an expression vector. Increased expression of thromboxane synthase in DUl45 cells was found to stimulate cell migration. Inhibition of thromboxane synthase or blockade of thromboxane A2 function inhibited prostate cancer cell migration. Our study suggest that thomboxane synthase and its eicosanoid product play a contributory role in prostate cancer progression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA410788
Entities
People
- Daotai Nie
- David Grignon
- Graham Pidgeon
- Kenneth V. Honn
- Mario Lamberti
Organizations
- Wayne State University