Rapid Screen for Tumor Suppressor Genes on Chromosome 19p13
Abstract
We have recently found that a region on chromosome 19p13 displays an unusually high rate of allelic imbalance (LOH) . In 78% of microdissected breast cancer specimens one allele copy was lost. The LOH rate at this locus is highest at the polymorphic marker D19S216, and is lower at nearby up- and downstream markers. Such loss is a hallmark of tumor suppressor genes. Although we are currently investigating one promising candidate for a tumor suppressor gene at this locus (named HET/SAF-B), we expect that this "hot spot" LOH region harbors more than one critical gene. This sort of clustering has been seen in other regions of high LOH; for example, a very small region on chromosome l3q (<25 cM) harbors a series of interesting genes including BRCA2, Brush1, FKHR, and Rb. We propose to use a new method to rapidly screen the 19p13 region for other candidates.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2002
- Accession Number
- ADA410864
Entities
People
- Steffi Oesterreich
Organizations
- Baylor College of Medicine