Regulatory Control of Breast Tumor Cell Poly (ADP-Ribose) Polymerase

Abstract

We have previously isolated an intact, stable, and fully functional multiprotein DNA replication complex (designated the DNA synthesome) from a variety of non-malignant and malignant tumor cells including breast cancer cells. We have shown that poly(ADP-ribose) polymerase PARP is among the components of the DNA synthesome. The transformation of a non-malignant human breast cell to a malignant state was accompanied by a significant alteration in the 2-D PAGE profile of specific protein components of the DNA synthesome (such as PCNA) together with a 6-8 decrease in the replication fidelity of the DNA synthesome.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2002
Accession Number
ADA410880

Entities

People

  • Linda Milkas
  • Waleed Abdel-aziz

Organizations

  • Indiana University Bloomington

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antigens
  • Biochemistry
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Chemistry
  • Electronic Mail
  • Mass Spectroscopy
  • Materials
  • Molecular Weight
  • Neoplasms
  • Phosphoamino Acids
  • Proteins
  • Two Dimensional

Fields of Study

  • Biology
  • Chemistry

Readers

  • Molecular Biology and Genetics