A Genomics Approach to Tumor Gemome Analysis
Abstract
Genomes of solid tumors are often highly rearranged and these rearrangements promote cancer progression through disruption of genes mediating immortality, survival, metastasis, and resistance to therapy. A growing number of these genes are targets for anti-tumor therapeutics. End Sequence Profiling (ESP) is a sequence-based method capable identifying all known structural rearrangements in a tumor genome with a resolution of 5kb for approximately 1% the cost of whole genome sequencing. Because ESP is sequence- based it can readily integrate expression profiling and proteomic data. Analysis of the breast cancer cell line MCF7 using ESP revealed a far more different structure of rearranged genome than previously appreciated. Our observations reveal colocalization of amplified loci, inversions, translocation breakpoints and provide evidence for packaging of cancer related genes in complex tumor-specific structures. The results have important implications for understanding tumor evolution and identification of novel biomarkers and targets for development of therapeutics.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2002
- Accession Number
- ADA410900
Entities
People
- Colin C. Collins
Organizations
- University of California, San Francisco