Structure/Function Studies of the Androgen Receptor DNA-Binding Region

Abstract

In this annual report we discuss the progress over the past 12 months towards characterizing the structure-function relationship in the androgen receptor (AR) DNA-binding domain (DBD). We have over-produced large quantities of the AR-DBD using E. coli expression systems, and obtained pure preparations of DNA targets of various size and composition, all of which incorporate the AR binding site. We continue to work towards obtaining the highest resolution X-ray diffraction data from crystals of the AR-DBD/DNA complex and have been optimizing our crystalline preparations. In addition, we have made progress toward characterizing previously unknown functions of the AR-DBD. We report in two manuscripts completed in the previous 12 months (included in the appendix) our findings that the DBD acts as the nuclear export signal for AR and related nuclear receptors, and that this export is dependent on Calreticuling and calcium.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2002
Accession Number
ADA411246

Entities

People

  • Fraydoon Rastinejad

Organizations

  • University of Virginia

Tags

DTIC Thesaurus Topics

  • Albumins
  • Androgen Receptors
  • Biomedical And Dental Materials
  • Carrier Proteins
  • Cell Line
  • Cells
  • Chemistry
  • Diffraction
  • Digital Images
  • Enzyme Inhibitors
  • Genetics
  • Health Services
  • Polymeric Films
  • Proteins
  • Proteomics
  • X Rays
  • X-Ray Diffraction

Readers

  • Clinical Trial Research.
  • Molecular and Cellular Biochemistry