Membrane-Bound Hyaluronidase in Breast Cancer Progression
Abstract
Using highly purified sperm hyaluronidase and PH-20 cDNA transfection approaches, we have demonstrated that: PH-2O HAase can release FGF2, stimulates the growth of both tumor cells in both anchorage -dependent and independent conditions. It can also stimulate the proliferation of endothelial cells and enhance the tumor angiogenesis. As the result of the dual effect, PH-20 HAase promotes the tumor growth in vivo. The results of in situ hybridization and immunohlstochemistry staining indicate that both mRNA and protein of PH-20 HAase are expressed in breast cancer cells. While there is little staining in normal breast tissues, there is a strong staining in breast cancer cells. The expressing rate increases from primary breast tumors without metastasis' (48.2%) to those with metastasis (70.8%) to those lymph node metastasis (83.3%), strongly suggesting that the PH-20 HAase is utilized by malignant breast cancer cells to promote their progression. The detection of the level of PH-20 HAase may serve as a poor prognosis index for human breast cancer. The reduction of the expression or the function of PH-20 HAase by genetic modification (anti-sense, ribozymes or siRNA) or small molecules may be an novel approach in anti-breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2002
- Accession Number
- ADA411263
Entities
People
- Lurong Zhang
Organizations
- Georgetown University