Expression Profiling of Cell Lines Expressing Regulated NF2 Transcripts
Abstract
Expression profiling is a powerful novel technique to examine changes in the expression of a large number of genes at the same time. Parallel analysis of gene expression reflects the changing view of signaling pathways towards signaling networks that have multiple and complex feedback and feed forward loops. Different phenotypic states of a cell can be translated into specific gene expression signatures. Expression profiling is not restricted to known genes, but changes in the expression of genes without known function can also be detected. As a complement to yeast two-hybrid studies we proposed using gene expression profiling to determine changes in gene expression as a function of NF2 expression in schwannoma cells. The strength of our approach is that we will not use tissues from patients, but will concentrate on cell lines in which NF2 expression can be controlled through the Tet/On system. We have now generated several cell lines that express NF2 in a regulated fashion. The parent lines are RT4 schwannoma cells and mouse embryonic fibroblasts. A time course for NF2 expression has been established. A total of four cell lines have been tested on microarrays to detect expression changes. Surprisingly, no changes common to expression of isoform 1 and 2 have been detected so far. We are currently in the process of repeating the experiments with slightly altered conditions to enhance the reliability of the detected expression changes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2002
- Accession Number
- ADA411281
Entities
People
- Stefan M. Pulst
Organizations
- Cedars-Sinai Medical Center