Alpha (1, 3) Galactosyltransferase Gene Therapy for Breast Cancer
Abstract
ALPHA(1,3)GALACTOSYL EPITOPES (ALPHA Ga1) expressed on membrane glycoproteins and glycolipids are the major target of the human hyperacute rejection response observed when organs are transplanted from nonprimate donor species. Expression of the alpha(1,3)galactosyltransferase gene ALPHA(1,3)GT by breast cancer cells will lead to their direct in vivo destruction by anti-alpha Ga1 antibodies and complement, and boost the immune response to other tumor antigens. In vitro transduction of MCF-7, and T47D human breast cancer cells with an HSV amplicon vector resulted in expression of the suicide gene as detected by specific binding of labeled IB-4 isolectin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA411322
Entities
People
- Charles J. Link