The Functional Role(s) of Neurofibromin During Neural Crest Cell Development
Abstract
This grant proposal set out to determine if neurofibromin plays an important role during early neural crest cell development The objectives of this study were: A) set-up and maintain a viable colony of Nf +/- mice to generate Nf +/+, Nf +/- and Nf -/- embryos. B) Set up a breeding protocol that provides accurately timed pregnant E9 embryos. C) Devise assays to reproducibly assay neural crest cell proliferation and cell death. D) Fix, freeze and collect enough E9 Nf +/+, Nf +/- and Nf -/- embryos with which to generate the relevant data. E) Serially section the embryos in D and perform the proliferation and cell death assays in C to determine whether or not neurofibromin modulates neural crest cell proliferation and cell death in these embryos. To date objectives A-D have been accomplished. Objective E is ongoing and should be completed over the next 6-9 months.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2002
- Accession Number
- ADA411420
Entities
People
- Peter G. Bannerman
Organizations
- Children's Hospital of Philadelphia