DNA Vaccination of Overcome Peripheral Tolerance

Abstract

The vaccination efficacy of DNA encoding autologous rat ErbB-2 (neu) or heterologous human ErbB-2 (Her-2) is compared in rat neu transgenic mice (BALB neuT). Cross reactivity between Her-2 and rat neu was tested initially by immunizing normal mice, twice, i.m. with pEFBosGM-CSF and pCMVE2TM or pCDneuTM. E2TM and neuTM encodes the extracellular (ECD) and transmembrane (TM) domains of Her-2 and neu, respectively. Immunized mice were challenged with mammary tumor D2F2 expressing Her-2 (D2F2/E2) or neu (D2F2/neu). All mice immunized with E2TM or neuTM rejected tumors expressing the corresponding antigen.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2002
Accession Number
ADA411427

Entities

People

  • Wei-Zen Wei

Organizations

  • Wayne State University

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Antibodies
  • Antigens
  • Biomedical Research
  • Cell Line
  • Cells
  • Coding
  • Immune System Phenomena
  • Immunity
  • Immunization
  • Immunogenicity
  • Immunomodulation
  • Lymphocytes
  • Reactivities
  • Vaccination
  • Vaccines

Fields of Study

  • Biology

Readers

  • Immunology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech