Biological Agent Sample Preparation for the Detection and Identification of Multiple Agents by Nucleic Acid-Based Analysis
Abstract
We have designed, developed and tested an instrument prototype for automating the "front end" of nucleic acid test procedures. The AutoLyser instrument which we have developed, provides fully automated purification of viral, bacterial and human genomic DNA and RNA from clinical samples, cell culture and swabs in as little as 10 minutes, and is capable of purifying between 1 and 8 samples at a time. No manual steps are required during a purification run. Samples are rendered non-infectious and are processed inside a disposable plastic device which can be quickly attached or removed from the instrument by relatively unskilled technical personnel. The possibility of contact with an infectious sample is almost eliminated. Centrifugation is not required since reagents are moved within the sample cartridge by means of precision syringe pumps and reagent selection valves under software control. Purified nucleic acid template is eluted in water and is ready for use in PCR or other downstream operations.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2002
- Accession Number
- ADA411664
Entities
People
- Robert Fields