Can Labeled Human CD 34+ Lymphocytes be Used to Identify Sites of Angiogenesis Within Growing Tumors

Abstract

We have proposed that CD34+ vascular endothelial progenitor (VEP) cells in blood can be used to transport therapeutic genes into areas of angiogenesis within growing breast cancer tumors. Our system is tumors (MDA-MB 231) grown in SCID mice. It was difficult to grow these tumors. We learned that NK cells and NKT cells had to be eliminated before tumors could grow. CD34+ cells were isolated from leukopacks and labeled with a lipophilic fluorescent dye, DiI. Leukopacks do not supply us with enough CD34+ cells to further isolate subsets of cells that have phenotypic markers of VEP.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2002
Accession Number
ADA411739

Entities

People

  • Stephen P. Brooks

Organizations

  • University at Buffalo

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Anatomy
  • Angiogenesis
  • Biological Sciences
  • Biomedical Research
  • Breast Cancer
  • Cells
  • Classification
  • Fluorescent Dyes
  • Hemic And Immune Systems
  • Information Operations
  • Lymphocytes
  • Neoplasms
  • New York
  • Security

Fields of Study

  • Biology
  • Medicine

Readers

  • Materials Science and Engineering.
  • Oncology (Cancer Research).