USF-1 as an Inhibitor of Mammary Gland Carcinogenesis

Abstract

The hypothesis tested in these studies is that overexpression of USF in the mammary glands of transgenic mice will inhibit myc-dependent tumorigenesis. Expression of a FLAG-tagged form or USF-2 was targeted to the mammary gland under the control of the mouse mammary tumor virus (mmtv) long terminal repeat. Of eight lines of transgenic mice that were generated, one demonstrated expression of flag-tagged USF-2 in the lactating mammary gland at levels 12-fold over that of endogenous USF-2. Evaluation of tumorigenesis in these mice (n=17) out to 453 days of age suggests that USF-2 when overexpressed by itself is not oncogenic. In contrast, 72% of mmtv-myc mice (n=18) analyzed have developed mammary tumors with an average latency of 156+/-33 days.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2002
Accession Number
ADA411741

Entities

People

  • Darryl L. Hadsell

Organizations

  • Baylor College of Medicine

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Apoptosis
  • Biological Staining And Labeling
  • Biomedical Research
  • Breast Cancer
  • Cell Nucleus
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Frequency
  • Glands
  • Information Operations
  • Inhibitors
  • Mammary Glands
  • Neoplasms
  • Terminals
  • Tissues

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics