Soft Lithography for Oligonucleotide Arrays Fabrication

Abstract

A method for fabricating high-density oligonucleotide arrays directed by a set of molecular stamp was reported in this paper. In this method, based on the standard phosphoramidites chemistry protocol, the coupling was conducted under soft lithography. A 20-mer DNA microarray with 10,000 probes has been successfully fabricated, which shows the advantages of accurate, reliable operation and low-cost production of high-density DNA chip.

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Document Details

Document Type
Technical Report
Publication Date
Oct 25, 2001
Accession Number
ADA411832

Entities

People

  • N. Y. He
  • P. F. Xiao
  • Q. G. He
  • Z. H. Liu
  • Zs. C. Liu

Organizations

  • Southeast University

Tags

DTIC Thesaurus Topics

  • Acetonitrile
  • Chemical Synthesis
  • Chemistry
  • Construction
  • Couplings
  • Dna Microarrays
  • Education
  • Fabrication
  • Fluorescence
  • High Density
  • Hybridization
  • Lithography
  • Nitriles
  • Organic Chemistry
  • Soft Lithography
  • Standards
  • Substrates

Fields of Study

  • Chemistry

Readers

  • Molecular Genetics
  • Nanofabrication and Microfabrication.