Effects of Surface Chemistry and Protein Competitive Adsorption on Cell Adhesion

Abstract

The competitive adsorption between collagen and bovine serum albumin (BSA) was investigated by imaging ellipsometry. The competitive adsorption of the binary solution depended on the surface chemistry of substrates, At the range of concentration investigated, collagen preferentially adsorbed on the hydroxy surface (-OH surface) but BSA on the methylic surface (-CH3 surface), ROS 17/2.8 cell adhesion on the two kinds of surfaces with or without the pre-adsorption of protein was also investigated, The results showed that the competitive adsorption caused ROS 17/2.8 cells preferentially adhered and spread well on the -OH surface, but less adhered and didn't spread on the -CH3 surface.

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Document Details

Document Type
Technical Report
Publication Date
Oct 25, 2001
Accession Number
ADA411905

Entities

People

  • G. Jin
  • P. Q. Ying
  • Z. L. Tao

Organizations

  • Chinese Academy of Sciences

Tags

Communities of Interest

  • Advanced Electronics

DTIC Thesaurus Topics

  • Adhesion
  • Adsorption
  • Cell Physiological Processes
  • Cells
  • Charge Coupled Devices
  • Chemical Synthesis
  • Chemistry
  • Collagen
  • Culture Techniques
  • Cultured Cells
  • Detection
  • Gray Scale
  • Imaging Techniques
  • Incubation
  • Numbers
  • Proteins
  • Surface Chemistry

Readers

  • Environmental Engineering
  • Immunology
  • Nanofabrication and Microfabrication.