Detection of Mitochondrial DNA Mutations in Mammary Epithelial Cells in Nipple Aspirate Fluid
Abstract
Because optimal methods have not been established for screening and early detection of breast cancer in premenopausal women, there is an urgent need for a variety of new approaches that can augment mammographic screening. We propose to study a new approach to early detection, based on mitochondrial DNA (mtDNA) mutation analysis of mammary epithelial cells shed into nipple aspirate fluid (NAF) and the Ductal Lavage (DL) fluid. The ability to detect these mutations in NAF and DL fluid, indicative of the early stages of breast cancer, would provide an important early detection approach that could complement conventional screening. Recent studies have shown the presence of several mtDNA mutations in a variety of tumor types, including colorectal, bladder, head and neck, and lung tumors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2002
- Accession Number
- ADA412041
Entities
People
- Bassem R. Haddad
Organizations
- Georgetown University