Does the Phenotyping of Disseminated Prostate Cancer Cells in Blood and Bone Marrow Prior to Radical Prostatectomy Provide Prognostic Information?
Abstract
Approximately 20% of men who undergo a radical prostatectomy later relapse with bone metastases. The cellular events that are predictive of subsequent progressive disease remain unknown. We've focused attention on the detection of disseminated CaP cells in the blood and bone marrow. Our hypothesis is that these disseminated cells may provide critical insight regarding biomarkers of use in prognostication. Enrichment and isolation methods developed over the past three years by us allows for the identification and study of these cells in enriched populations or as individual cells. Using these combined new technologies we've begun to phenotype the disseminated CaP cells in blood and bone marrow of fifty patients undergoing a radical prostatectomy and who are found to have a Gleason Sum of greater than 7. Cells are also being stored and upon PSA biochemical progression, a subset will be analyzed so that comparisons of pre-progression and post-progression phenotypes can be made on the same patient and contrasted to (a) the phenotypic profiles of his primary tumor and (b) a disseminated CaP set of cells isolated from the bone marrow of patients with advanced disease following radical prostatectomy. Unfortunately, enrollment of patients was delayed by nine months due to DoD human subject issues.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA412293
Entities
People
- Robert L. Vessella
Organizations
- University of Washington