Human Dermal Fibroblasts (Adult): Instructions for Initiation of Cultures from Cryopreserved Cells and Subculture

Abstract

This technical report outlines procedures that have been developed to provide a cost effective way to produce large quantities of normal human skin fibroblast cells (NHSFs) for studying the mechanism(s) of action of chemical warfare agents (CWAs) and medical counter-measurements against CWAs. Various growth factors were added to media recommended by the supplier, American Type Culture Collection (ATCC), Manassas, Virginia, USA. Cell viability, cell differentiation and cell proliferation were evaluated using different tissue culture assays in particular the CyQUANT(Registered) Cell Proliferation Assay kit obtained from Molecular Probes, Inc., Eugene, Oregon, USA. This particular assay measures cell proliferation and was used as indicated in the experimental protocol provided by the product brochure. We found cultured human skin cells to be a useful model for skin irritation testing.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2002
Accession Number
ADA412546

Entities

People

  • Carmen M. Arroyo
  • David W. Kahler

Organizations

  • United States Army Medical Research Institute of Chemical Defense

Tags

DTIC Thesaurus Topics

  • Biological Sciences
  • Biomedical Research
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Warfare
  • Chemical Warfare Agents
  • Culture Techniques
  • Environmental Pollutants
  • Fibroblasts
  • Growth Factors
  • Instructions
  • Measurement
  • Medical Personnel
  • Military Research
  • Particle Counters
  • Tissue Culture

Readers

  • Immunology and Pathology
  • Microbial Pathology
  • Trauma Surgery or Emergency Medicine.