Tumor Specific Gene Expression and Tumor Specific Vector Replication for Systemic Chemotherapy Sensitization Treatment of Breast Cancer
Abstract
Once breast cancer recurs after initial surgery or radiation therapy and is found to be incompletely responsive to salvage chemotherapy, there is no established treatment that can prolong survival. To address this problem, the Deisseroth laboratory has developed a series of vectors that are directed at the use of the L-plastin tumor-specific transcriptional promoter to control the expression of a chemotherapy sensitization gene (cytosine deaminase) and a viral replication gene (ElA) so that any toxic effect is tumor specific. These vectors have been shown to suppress the growth of human breast cancer cell lines in a SCID mouse model, and to produce a direct cytolytic effect on breast cancer cell lines which is not seen in explant cultures of normal breast epithelial cells. By combining the ElA and the CD transcription units in a single vector the authors have shown that this vector is superior to vectors with a single ElA or CD transcription unit. The authors will now complete the steps necessary for this vector to be available for therapy and will study the use of this vector in combination with chemotherapy as a means to directly kill breast cancer cells and to control metastatic breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA412572
Entities
People
- Albert B. Deisseroth