Development of a Diagnostic Blood Test for Breast Cancer
Abstract
The purpose of this study is to develop a diagnostic blood test for breast cancer. The concept is based on the premise that breast cancers shed sufficient quantities of DNA into plasma to be detected using methylation-specific PCR. During the second year of this study, a small number of serum samples from breast cancer patients were tested for methylation of genes previously determined to be methylated in the cancer tissue. Disappointingly, methylation was detected in only two of these serum samples. Therefore, in the third year of the project, we tested a highly sensitive method for detection of MSP products. Because the genes that we originally used to assay cancers for methylation showed positive results in relatively low - percentages of breast cancer cases, we also profiled methylation of a number of additional genes (12 in all) in over 100 breast cancer cases.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2002
- Accession Number
- ADA412743
Entities
People
- Edward W. Gabrielson
Organizations
- Johns Hopkins University