Novel Oncogenes in Breast Cancer Development
Abstract
Breast cancer is a multi-step genetic process involving mutations of oncogenes and tumor suppressor genes With a few notable exceptions the genetic determinants that contribute to the development of breast cancer remain unknown We have developed and applied a novel retrovirus-based library screening strategy coupled to a biological assay for growth transformation, to identify novel oncogenes in breast cancer development The approach involves the isolation of mRNA from noninvasive (MCF-7) and invasive (BT549 and Hs578T) human breast carcinoma cell lines to generate full length cDNA sequences, and introduced into a retrovirus expression vector The retrovirus, encoding genes expressed in breast cancers, is infected into Rat-l fibroblasts or RIE-l rat intestinal epithelial cells and screened for the appearance of foci of transformed cells The resulting transformed cells are isolated and the retrovirus-associated cDNA is rescued After sequence analyses, and verification of transforming activity of the isolated sequences, further analyses are done to assess the contribution of the isolate% genes to breast cancer development From these screens, we have identified the FGF receptor 2 and the Raf-l serine/threonine kinase.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA412903
Entities
People
- Chaning Der
- James J. Fiordalisi
Organizations
- University of North Carolina at Chapel Hill