Blood-Brain Barrier Transport of Uranium
Abstract
During the first 9 months of work, studies were directed at (1) characterizing the suitability of immortalized Rat Brain Endothelial 4 (RBE4) cells as a model for in vitro blood-brain barrier (BBB) carrying (2) Transfection of RBE4 cells and Chinese Hamster Cells (CHO) with the Divalent Metal Transporter-i (DMT-l) gene to enable future studies on the role of this transport in uranium transport (3) Measurements of uranium uptake in RBE4 cells with Neutron Activation Analysis (NAA) The results presented in this report establish (1) the utility of the model to transport uranium Treatment of RBE4 cells with Astrocyte Conditioned Medium (ACM) endows the BBB restrictive properties and alters DMT-l expression profiles, while restricting the transport of polar substances, such as inulin (2) RBE clones and CHO cells have been successfully transfected, and are presently being sequenced to confirm that they match the original DMT-l Currently, transfected CHO cells have been selected with 4-18 antibiotic and the forward clones appear to be expressing more DMT-l protein than reverse or control cells by western blot The transfected RBE4 cells are in the selection process and will be analyzed as soon as there are enough G4-lB resistant cells (3) Initial NAA measurements confirm the uptake of uranium into endothelial cells
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2002
- Accession Number
- ADA412998
Entities
People
- Michael Aschner
Organizations
- Wake Forest University