Impact of Disrupted Brca2 Protein-Protein Interactions on DNA Repair and Tumorigenesis

Abstract

In this report, we have investigated the effects of overexpression of active Ras within the mammary epithelium. Using the tetracycline-inducible system, we have generated a line of mice which, when also bearing rtTA transgene expressed under control of the MMTV LTR, inducibly express active H-Ras in the mammary epithelium in response to the drug doxycycline. We have shown that the mammary epithelium undergoes a large amount of proliferation in response to Ras Removal of doxycycline abrogates the hyperplastic phenotype and restores a normal gland within 60 days. We have also shown that pathways downstream of Ras are activated, such as the Raf/MEK/MAPK pathway. Furthermore, the pl9/p53/p21 pathway is also activated in response to Ras.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2002
Accession Number
ADA413006

Entities

People

  • Christopher J. Sarkisian
  • Lewis A Chodosh

Organizations

  • Drexel University

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Biological Aging
  • Biomedical Research
  • Cell Line
  • Cells
  • Cultured Cells
  • Deoxyribonucleic Acids
  • Epithelial Cells
  • Epithelium
  • Genetics
  • Laboratory Animals
  • Mammary Glands
  • Materials
  • Phenotypes
  • Protein-Protein Interactions
  • Proteins
  • Tissues

Fields of Study

  • Biology
  • Medicine

Readers

  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech