Markers of Increased Risk in Pre-Invasive Breast Cancer
Abstract
The risk of recurrence and progression of ductal carcinoma in-situ (DCIS) of the breast is best designated by morphological indicators including the presence of necrosis. To identify molecular alterations underlying this morphological feature we have compared gene expression within a cohort of 6 cases of DCIS with necrosis (DCIS necrosis+) and 4 cases without necrosis (DCIS necrosis-) using microdissection and cDNA microarray. A set of 69 cDNAs, from a group of 1,181, was identified that were consistently differentially expressed. Amongst this set, the mRNA for angio-associated migratory cell protein (AAMP) and a serine threonine protein kinase, nuclear Dbf2 related (NDR), were consistently higher in DCIS necrosis+ and were also found to be overexpressed in the T47D breast cancer cell line subjected to hypoxia. A significant differential expression (P=0.0095) was confirmed for AAMP by quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Further study of AAMP by RT-PCR and in situ hybridization analysis of 37 cases of DCIS also confirmed that a higher AAMP mRNA expression tended to be associated with high-grade nuclear morphology and necrosis. The present study shows that while levels of gene expression are mostly similar between morphologically different DCIS, consistent differences in expression of a subset of genes can be identified between DCIS with and without necrosis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2003
- Accession Number
- ADA413543
Entities
People
- Adewale Adeyinka
- Peter H. Watson
- Soma Mandel
Organizations
- University of Manitoba