X-Ray Crystallography of Botulinum Neurotoxin Type E and Its Functional Subunits

Abstract

Botulinum Neurotoxin type E was crystallized and an electron density map was analyzed at 3 alpha resolution. A new crystal form was also obtained, and may be helpful in improving the phasing. Co-crystallization experiments with gangliosides have also been undertaken. The structure of a complex between tetanus toxin C-fragment and doxorubicin has been determined, and drug binding occurs at the ganglioside binding site. Structure determination of a complex between tetanus toxin C fragment and the ganglioside GDlb is underway, and data have been collected.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2002
Accession Number
ADA413858

Entities

People

  • Martin Sax
  • S. Swaminathan
  • William Furey

Organizations

  • United States Department of Veterans Affairs

Tags

DTIC Thesaurus Topics

  • Chemistry
  • Crystal Structure
  • Crystallization
  • Crystallography
  • Crystals
  • Data Sets
  • Diffraction
  • Electron Density
  • Electrons
  • Electrospray Ionization
  • Materials
  • Molecules
  • Neurotoxins
  • Radiation
  • Sialic Acids
  • X Rays
  • X-Ray Crystallography

Fields of Study

  • Chemistry

Readers

  • Materials Science and Engineering.
  • Microbial Pathology
  • Systems Analysis and Design

Technology Areas

  • Microelectronics