Oxidized DNA Bases in Breast Cancer

Abstract

Recently more studies have correlated the elevated 5-hydroxymethyl-2'-deoxyuridine (HMdU - an oxidized DNA base) levels with an increased risk of cancer when compared to controls. HMdU has been proposed to be a biomarker for breast cancer risk Although methods are available, but requires larger sample sizes (between 50- 100 micrograms DNA), which limits its application for HMdU quantitation in relatively smaller sample size We have developed a sensitive method to detect and quantitate HMdU using fluorescent coumarin derivative to label HMdU and other nucleosides.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2002
Accession Number
ADA413879

Entities

People

  • Krystyna Frenkel
  • Mehul Desai

Organizations

  • New York University

Tags

DTIC Thesaurus Topics

  • Biological Markers
  • Biomedical Research
  • Blood Cells
  • Blood Coagulation
  • Breast Cancer
  • Cells
  • Chlorides
  • Coumarins
  • Detection
  • Detectors
  • Flow Rate
  • Fluorescence
  • Laser Dyes
  • Molecules
  • Neoplasms
  • New York
  • Nucleosides

Readers

  • Aerosol Science/Aerosol Physics
  • Chemistry (specifically Chemical Fluorescence)
  • Oncology and Biomarker-Based Cancer Detection.