Non-Invasive Diagnosis of Androgen Sensitivity in Human Prostate Tumors
Abstract
We characterized human prostate tumors by histology and quantitative NMR spectroscopy to determine if altered prostate gene expression produced NMR detectable changes in several metabolites (e.g., citrate, triacyiglycerols, taurine, tyrosine). These may provide sensitive and specific predictors of the presence, and the androgen sensitivity, of prostatic adenocarcinoma. Cur investigations provided a sound biochemical basis for the application of non invasive, in vivo NMR spectroscopy to assess tumor differentiation and androgen sensitivity in prostate cancer patients. We established a conventional tissue diagnosis by optical histology and computer-aided measurement of epithelial area, on the NMR tissue samples, assigned a Gleason score to each sample, and performed in vitro quantitative lH and 13c NMR spectroscopic measurements of the metabolic markers on tissue samples from human prostate tumors and adjacent non tumor tissue. Computer based Linear Discriminant Analysis will be used to develop a discrimination function based on the NMR spectroscopic measurements, the histology, Gleason score, and PSA measurements, and the patient's progress, to determine the most specific and sensitive NMR spectroscopic predictors of tumor diagnosis, grade, and hormonal sensitivity. We then applied NMR spectroscopy to human patients to measure the metabolites determined above as the most sensitive and specific predictors of grade, stage and hormonal responsiveness, and will compare the measurements with patient outcomes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2003
- Accession Number
- ADA413943
Entities
People
- Laurel O. Sillerud
Organizations
- University of New Mexico