Functional Analysis of C-CAM1 Tumor Suppressor Gene by Targeted Gene Deletions
Abstract
C-CAMl (renamed CEACAM1) is a cell adhesion molecule of the immunoglobulin supergene family. We have shown that CEACAM1 plays critical roles in prostate cancer initiation and progression. We propose to determine the functional roles of CEACAMl in normal prostate development, prostate homeostasis and prostate tumorigenesis by using gene targeting technologies to generate CEACAM1 knockout mice. We have constructed a Ceacam1 targeting vector in which Ceacam1 gene was flanked by loxP sites to allow for generation of both straight and conditional knockout of the Ceacam1 gene. Several embryonic stem cell clones containing the recombinant gene allele were established and two were injected into blastocysts for germ line transmission of the targeting construct. In this funding period, we have succeeded in producing mice harboring the conditional Ceacam1 knockout allele. Two founder mice from two embryonic cell clones showed germ line transmission of the targeting construct containing the conditional Ceacam1 gene. Five mice with homozygous conditional Ceacam1 alleles were born from these two founder mice. These results suggest that embryonic lethality does not occur in the mice carrying conditional Ceacam1 knockout alleles, thus it is possible to pursue the function of CEACAMl in normal prostate development, prostate homeostasis and prostate tumorigenesis in vivo.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2003
- Accession Number
- ADA414431
Entities
People
- Sue-hwa Lin
Organizations
- The University of Texas MD Anderson Cancer Center