Bioengineered Proteins for Chemical/Biological Defense, Protection and Decontamination
Abstract
Report developed under contract for STTR topic "ARMY01-T005". The purpose of the project was to demonstrate feasibility of developing transgenic chickens by methods invented by the Contractor and RI. The method involved construction of a patented transposon-based genetic vector and injecting this into the testes of young roosters. A total of 88 white leghorn roosters underwent surgery and had the vector injected (^5 microgram DNA) into their testes at 4-14 weeks of age. Upon attainment of sexual maturity at ^20 weeks a spermatozoa sample was obtained and PCR analysis performed to ascertain incorporation of the gene. Confirmed 'transgenic spermatozoa were detected in eight mature roosters (^9%) which demonstrates feasibility of the method. These 'transgenic' roosters could be expected, by simple breeding, to generate female offspring also carrying the gene. This makes possible the prospect of gene pharming' from the white of eggs from hens carrying a transgene coding for a variety of pharmaceuticals or peptides.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 14, 2002
- Accession Number
- ADA414539
Entities
People
- Kenneth F. Deboer
- Richard K. Cooper