The Development of BRCA2-Deficient Mice
Abstract
Women who inherit a BRCA1 or BRCA2 mutation have an 80-90% chance of developing breast cancer. Gene targeting techniques were used to create a Brca2-deficient mouse. A portion of exon 10 was replaced with the Neo gene resulting in premature truncation of the protein product. Examination of normal mammary ductal development in 129(+/+) and 129(+/Brca2-) mice revealed phenotypic differences between the two genotypic classes. 129(+/+) and 129(+/Brca2-) mice and several inbred mouse strains were used to study the effect of genetic background on radiation induced mammary tumor induction. BALB/c and SWR mice are susceptible to radiation induced mammary tumorigenesis and C57BL/6, FVB/N and C3H mice are relatively resistant. While FVB/N mice do not develop tumors, radiation treatment has a dramatic effect on ductal morphogenesis. To date, no mammary tumors have been observed in the 129(+/+) and 129(+/Brca2-) mice. Brca2-deficient mice were crossed to p53 mutant mice to generate four genotypic classes of offspring. A study is in progress to assess the consequences of harboring mutation in the two tumor suppressor genes with and without radiation exposure. The mammary tumor susceptible BALB/c and resistant C57BL/6 mouse strains being used to make mice congenic for the Brca2 mutation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2002
- Accession Number
- ADA414587
Entities
People
- L. M. Bennett
- Roger Wiseman
Organizations
- Lawrence Livermore National Laboratory