Engineered Plants as Biosensors

Abstract

The aim of the research was the creation of a model biosensing plant that could detect plant diseases and to characterize the utility of laser induced fluorescence imaging for detecting the inducible (LIFI) plant signal. Tobacco was engineered with a plant pathogen inducible promoter (GN1) fused to a green fluorescent protein (GFP). GFP was upregulated in response to a salicylic acid simulant (BTH) and plant pathogens (PART 1). GFP fluorescence was low, but detectable. LIFI I/O was characterized for highly fluorescing transgenic plants (PART 2).

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Document Details

Document Type
Technical Report
Publication Date
May 28, 2003
Accession Number
ADA414723

Entities

People

  • C. N. Stewart Jr.
  • Mentewab Ayalew
  • Mitra Kooshki

Organizations

  • University of Tennessee system

Tags

Communities of Interest

  • Sensors

DTIC Thesaurus Topics

  • Abstracts
  • Biosensors
  • Detection
  • Detectors
  • Fluorescence
  • Fungi
  • Gene Expression
  • Genetically Modified Organisms
  • Laser Induced Fluorescence
  • Lasers
  • Pathogenic Bacteria
  • Remote Sensing
  • Salicylic Acids
  • Spectra
  • Spectroscopy
  • Standards
  • Tobacco Plants

Readers

  • Analytical Chemistry
  • Energy Conservation and Renewable Energy Engineering.
  • Molecular Genetics

Technology Areas

  • Biotechnology
  • Directed Energy