Gene Therapy for Prostate Cancer Radiosensitization Using Mutant Poly (ADP-Ribose) Polymerase
Abstract
Poly.(ADP-ribose) polymerase (PARP) has strong affinity for DNA strand breaks and cycles on and off the DNA ends to allow DNA repair. A DNA-binding domain of PARP (PARP-DBD) acts as a dominant-negative mutant by binding to DNA strand breaks irreversibly and sensitizing mammalian cells to DNA-damaging agents. Therefore, expression of PARP-DBD in prostate carcinoma cells offers a strategy, to achieve sensitization to genotoxic treatments. Toward this end, we developed recombinant plasmids expressing the PARP-DBD under the control of the 5, -flanking sequences of the human prostate-specific antigen (PSA) gene.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2003
- Accession Number
- ADA414783
Entities
People
- Viatcheslav A. Soldatenkov
Organizations
- Georgetown University