The Role of p53 Mutations in Metastasis of Prostate Cancer to Bone
Abstract
The mortality and morbidity of prostate cancer (CaP), the commonest cancer in men in Western society, are largely caused by the spread of CaP to bone How this occurs, and the interactions between CaP cells and bone are poorly understood Specific mutations of the tumor suppressor gene, p53, that occur in CaP may cause disease progression To test this, we have generated cell lines derived from the human LNCaP cell line that stably express wild type or mutant p53. Using generated lines, to test whether p53 mutations affect establishment and growth of experimentally-induced CaP in the bone. Our initial studies in tissue culture will show that factors produced by the variant LNCaP lines may alter the normal processes of bone remodeling and angiogenesis. No effects were seen on the proliferation of bone forming cells; those on cellular differentiation are currently under study We have perfected the technique for implanting CaP cells in the bone of immunologically deficient mice, and established a "take rate" for some LNCaP lines. Further studies will provide information about how specific mutations of p53 found in patients with clinical disease impact on progression, and could lead to the development of new therapeutic strategies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2003
- Accession Number
- ADA414821
Entities
People
- Barbara Szymanska
- Elizabeth A. Kingsley
- Janna E. Quinn
- Julie M. Brown
- Pamela J. Russell
Organizations
- University of New South Wales