Sequence of Spider Aciniform and Piriform Silks

Abstract

The aciniform glands from Argiope Trifasciata were used to construct a cDNA library. The library was probed with various DNA probes based on known spider silk protein sequences. A large 9kb clone was identified and partial sequencing has been done. The C-terminal sequence is clearly related to the same sequence in a number of other spider silks. The 5' end of the clone still appears to be repetitive sequence and thus it is unlikely to be a full-length clone. We have completed about 4.5 kb of sequence at this point. The remaining sequence is being completed. There were an additional clones of smaller length found but all contain the same sequence as the largest one. The sequence to date shows some homology to other spider silks but is distinctly different. It appears to have a repeat sequence of a cDNA library from Nephila clavipes has been constructed from the aciniform/piriform glands and is in the process of screening. This will be used to compare the aciniform silk sequences as well as to identify the piriform silk sequences.

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Document Details

Document Type
Technical Report
Publication Date
Sep 19, 2001
Accession Number
ADA414965

Entities

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  • Randolph V Lewis

Organizations

  • University of Wyoming

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  • Human Systems

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Biopolymers
  • Classification
  • Dna Sequence Analysis
  • Genetic Structures
  • Information Operations
  • Mathematics
  • Military Research
  • Nucleic Acids
  • Proteins
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