DNA Replication During Conjugal Transfer of R1162

Abstract

Conjugative DNA transfer is an important mechanism for the acquisition of multiple drug resistance by bacteria. The role of DNA synthesis in this process was examined under conditions where vegetative replication was strictly prevented. There was more than one round of transfer from a donor cell containing a single plasmid molecule, indicating replacement strand synthesis by a conjugation-specific mechanism. A significant time lag between rounds of transfer was detected, arguing against the spooling of DNA into recipients by a rolling-circle replication.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2002
Accession Number
ADA415172

Entities

People

  • Richard Meyer

Organizations

  • University of Texas at Austin

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acids
  • Anti-Bacterial Agents
  • Bacteria
  • Bacterial Infections
  • Biology
  • Chemical Reactions
  • Chemistry
  • Deoxyribonucleic Acids
  • Escherichia Coli
  • Gel Electrophoresis
  • Genetics
  • Gram-Negative Bacteria
  • Microbiology
  • Mobilization
  • Molecular Biology
  • Molecules
  • Polymerase Chain Reaction

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular Genetics