Nanoparticle-Mediated p53 Gene Therapy for Breast Cancer

Abstract

The effect of different formulation parameters on nanoparticle-mediated gene transfection in vitro was studied Nanoparticles encapsulating plasmid DNA encoding fire fly luciferase were formulated using poly lactide (PLA) and poly dI-lactide co-glycolide (PLGA) polymers of different composition and molecular weight A multiple emulsion solvent evaporation method with polyvinyl alcohol (PVA) as an emulsifier was used to formulate DNA-loaded nanoparticles Transfection of various nanoparticles formulations was determined in breast cancer (MCF-7) cell line. Nanoparticles formulated with PLA demonstrated relatively lower transfection than those formulated with PIGA because of higher DNA release from PLGA nanoparticles than that from PLA nanoparticles.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2003
Accession Number
ADA415301

Entities

People

  • Jamboor K. Vishwanathan
  • Swayam Prabha
  • Vinod D. Labhasetwar

Organizations

  • University of Nebraska Omaha

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Biomedical And Dental Materials
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Electron Microscopy
  • Gene Therapy
  • Hydrophilic Properties
  • Hydrophobic Properties
  • Liquid Chromatography
  • Materials
  • Particle Size
  • Peptides
  • Polymeric Films
  • Resins
  • Sodium Compounds
  • Therapy

Readers

  • Molecular Genetics
  • Nanocomposite Materials Science
  • Polymer Science and Engineering.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech