Endometase in Androgen-Repressed Human Prostate Cancer

Abstract

The spread of prostate cancer cells to other parts of the body is the leading cause of patient death. In 2000, we reported the discovery, cloning, and characterization of human matrix metalloproteinase-26 (MMP-26), endometase. We have been testing three specific hypotheses: 1) The expression levels of MMP-26 is correlated with the metastatic potentials and the degrees of malignancy of human prostate cells; 2)MMP-26 has unique structure and enzymatic function; 3) MMP-26 enhances prostate cancer invasion by digesting extracellular matrix proteins and inactivating serine proteinase inhibitors, and specific inhibitors of MMP-26 block prostate cancer invasion. We have showed that the levels of MMP-26 protein in human prostate carcinomas from multiple patients were significantly higher than those in prostatitis, benign prostate hyperplasia, and normal prostate glandular tissues. MMP-26 promoted prostate cancer invasion via activation of pro-gelatinase B/MMP-9. The endometase active site structure and function have been investigated using synthetic metalloproteinase inhibitors. These results suggest that endometases may be a novel marker for prostate cancer diagnosis and prognosis and a new target for prostate cancer therapy. More detailed results and summary are described in attached two J. Biol. Chem. papers published and in press from Dr. Sang's laboratory.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2003
Accession Number
ADA415315

Entities

People

  • Qing-xiang A. Sang

Organizations

  • Florida State University

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biochemistry
  • Cancer
  • Cells
  • Cellular Structures
  • Chemical Synthesis
  • Chemistry
  • Confocal Laser Scanning Microscopy
  • Confocal Microscopy
  • Culture Media
  • Enzyme Inhibitors
  • Liquid Chromatography
  • Mass Spectrometry
  • Neoplasms
  • Peptides
  • Statistical Analysis
  • Tissues

Fields of Study

  • Biology
  • Medicine

Readers

  • Breast cancer cell signaling and growth regulation.
  • Oncology (Cancer Research).