Prevention of Development of Recurrent Growth of Prostate Cancer
Abstract
The purpose of the proposed studies is to identify and then target one gene or a small number of genes critical for the development of recurrent growth of CaP. Differential expression analysis, subtractive hybridization and immunohistochemistry were used in the androgen-dependent human prostate cancer CWR22 model to identify 10 genes whose expression might be associated with the onset of CaP recurrence. In the first year of the proposed studies, we have visually scored 8 gene proteins in CWR22 tumors on day 120 after castration and identified Nkx3.l, Q-tubulin and IGFBP-5 as potential targets. In order to more rigorously test these gene targets, we developed a hybrid immunostaining protocol for comparison of expression of antigens in proliferating versus non-proliferating cells. We have collected the necessary reagents (BrdU-labeled tumors), are modifying our image analysis algorithms to allow quantitation, have demonstrated the feasibility of an antisense approach and have begun to work with the serial prostate biopsies. We have made progress toward identifying targets for antisense therapy that will be tested in in vitro in CaP cell lines and in vivo in the CWR22 model.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2003
- Accession Number
- ADA415332
Entities
People
- James L Mohler
Organizations
- University of North Carolina at Chapel Hill