Novel Biosensors for the Detection of Biological Warfare Agents

Abstract

The specific aim of this project was to use the technique referred to as SELEX (Systematic Evolution of Ligands by EXponential enrichment) to generate stabilized RNA ligands ("aptamers") against the potential biowarfare agents Ricin A and Protective Antigen. SELEX experiments using RNA pools containing 2'-F substituted pyrimidine nucleotides were performed, and aptamers to both targets were generated. The Ricin A aptamer binds native, glycosylated, Ricin A with an equilibrium dissociation constant of approximately 60 nM. The aptamer also binds deglycosylated and recombinant Ricin A with similar affinity.

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Document Details

Document Type
Technical Report
Publication Date
Jun 16, 2003
Accession Number
ADA415590

Entities

People

  • Sharon Cload

Tags

DTIC Thesaurus Topics

  • Acids
  • Biological Warfare
  • Biological Warfare Agents
  • Biosensors
  • Biotechnology
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Detectors
  • Dissociation
  • Molecules
  • Nucleic Acids
  • Nucleotides
  • Pyrimidines
  • Sequences
  • Standards
  • Warfare

Fields of Study

  • Biology
  • Chemistry

Readers

  • Critical Infrastructure Protection in CBRN and WMD Threats.
  • Molecular Genetics

Technology Areas

  • Biotechnology