Retinol Esterification in Human Breast Cancer Cells

Abstract

This study was designed to identify the target genes of retinoids in the normal human mammary epithelial cells (HMEC) and breast cancer cells. In our early studies, we found that the IL-1Beta gene was a responsive gene to retinoic acid (RA) and its expression was up-regulated as early as two hours after RA treatment. In the recent studies, we examined the gene expression profiles in the human mammary epithelial cells following RA and 4-oxo-retinol (4-oxo-ROL) treatment. Our results indicated that both 4-oxo-ROL (1 micrometer) and RA (1 micrometer) strongly inhibited the proliferation of HMEC. The microarray analyses show that a large number of genes, including transcription factors, nuclear receptor co-regulators, cell cycle regulatory proteins and protein kinases, are regulated by RA and 4-oxo-ROL. Although many genes were found to be regulated by both RA and 4-oxo-ROL, 4-oxo-ROL was not converted to RA in HMEC, which suggests that 4-oxo-ROL regulates gene expression by a mechanism different from RA.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2003
Accession Number
ADA415600

Entities

People

  • Limin Liu

Organizations

  • Weill Cornell Medicine

Tags

DTIC Thesaurus Topics

  • Acids
  • Biological Factors
  • Breast Cancer
  • Carrier Proteins
  • Cell Physiological Processes
  • Cells
  • Epithelial Cells
  • Gene Expression
  • Growth Factors
  • Metabolism
  • Microarray Analysis
  • Neoplasms
  • New York
  • Peptides
  • Proteins
  • Retinoic Acids
  • Transcription Factors

Readers

  • Immunology and Pathology
  • Molecular Biology and Genetics