Prostatic Fluid Cells
Abstract
Most research that requires the long-term propagation of prostate cancer (PCA) cells in culture is carried out with three cell lines: DU 145, PC-3, and LNCaP. All of these lines were derived from metastases; only one, LNCaP, makes prostate specific antigen (PSA) and/or androgen receptor. Neither DU 145 nor PC-3 exhibit any phenotypic markers specific for prostatic epithelial cells. Better models are needed. There is evidence that some prostatic fluids contain PCA cells. For this proposal, our goals are (1) to test the tumorigenicity of and to develop transplantable xenografts from PCA cells in prostatic fluid, (2) to develop methods for enhancing the tumorigenicity of small numbers of these PCA cells without deliberately altering their genes, (3) to test these methods for enhancement of tumorigenicity with prostatic fluid cells, and (4) to initiate clinical follow-up. To date, we have detected significant, often sustained elevations of PSA in the blood of several mice that received prostatic fluid cells for many months after injection. In the case of two mice that received prostatic fluid cells from one patient, PSA increased progressively over the course of a year but started to fall at the 12-month time point. Cells from the injections sites were transplanted; one recipient has an elevated PSA. We have studied the coinjection of lethally irradiated, growth-factor- producing cells from several tumors with encouraging results in the case of some tumors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2003
- Accession Number
- ADA415846
Entities
People
- Thomas G. Pretlow
Organizations
- Case Western Reserve University