Autocrine and Paracrine Control of Breast Cancer Growth by Sex Hormone-Binding Globulin
Abstract
We propose that the expression of Sex Hormone-Binding Globulin (SHBG) by breast cancer cells is biologically regulated and that this SHBG functions to alter the effects of estrogens within the breast cancer cell. The expression of SHBG by breast cancer cells raises the important question of how the local regulation of SHBG synthesis and secretion affects both the sequestration of steroid hormones within the breast, and estrogen induced signal transduction at the cell membrane. The local synthesis of SHBG is consistent with an autocrine/paracrine role for this protein in breast cancer. We have made significant progress setting up our in vitro system for studying the effects of SHBG expression on breast cancer growth. We have generated candidate clones, derived from the human MCF-7 breast cancer cell line, that can be induced to either overexpress SHBG or to silence endogenous SHBG expression. These clones, along with a vector control cell line, will serve as vehicles to study the effects of SHBG expression on the regulation of steroid based signaling in human breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2003
- Accession Number
- ADA416108
Entities
People
- Scott M. Kahn
- Wiliam Rosner